RNA

Part:BBa_K4410001

Designed by: Ruyi Shi   Group: iGEM22_Worldshaper-HZBIOX   (2022-09-19)


Fragment H2 from E.coli Nissle 1917

This part is a down-stream sequence of argR gene from the genome of E. coli Nissle 1917. The argR gene knockout method is to use the λ-Red homologous recombination system, so it is necessary to add argR homology arms upstream and downstream of the replacement gene for subsequent gene knockout. This part is the downstream argR homology arm.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None